T4-phage deoxycytidylate deaminase is a metalloprotein containing two zinc atoms per subunit.
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چکیده
منابع مشابه
Protein-protein interactions involving T4 phage-coded deoxycytidylate deaminase and thymidylate synthase.
The enzymes deoxycytidylate deaminase (EC) and thymidylate synthase (EC) are functionally associated with one another, since they catalyze sequential reactions. In T4 coliphage infection the two enzymes are found in dNTP synthetase, a multienzyme complex for deoxyribonucleotide biosynthesis. Protein-protein interactions involving the phage-coded forms of these two enzymes have been explored in ...
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Deoxycytidylate (DCMP) deaminase was assayed at various times during and after normal and abnormal pregnancies. The level in amniotic fluid was assessed at induction and at caesarean section, and cord blood levels were estimated after normal delivery and at caesarean section. A rise occurred during labour and after hysterectomy and caesarean section--returning to normal after 2-3, and 12 days r...
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Evidence has been presented that chick embryo deoxycytidylate deaminase (EC 3.5.4.12) is capable of undergoing structural changes under a variety of conditions. Rate studies revealed that inhibition of the enzyme by ethylenediaminetetraacetate markedly diminishes at 10” and below. Although EDTA does not inhibit the deaminase at lo”, the inhibition by urea, N-ethyhnaleimide, and 5,5’dithiobis(Z-...
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During the infection by bacteriophage T4, dTMP and 5hydroxymethyl dCMP (HmdCMP) are synthesized in the ratio of 2:1, respectively, i.e. at the Thy/HmCyt ratio found in T4 DNA. This study demonstrates that on infection by a cd (phage-induced dCMP deaminase) mutant the dTMP/ HmdCMP synthesis ratio is approximately 0.6. The ratio remains about 0.6 on infection by cd dna double mutants. At the same...
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Although TroA (Tromp1) was initially reported to be a Treponema pallidum outer membrane protein with porin-like properties, subsequent studies have suggested that it actually is a periplasmic substrate-binding protein involved in the transport of metals across the treponemal cytoplasmic membrane. Here we conducted additional physicochemical studies to address the divergent viewpoints concerning...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1993
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)53770-4